I-cryopreservation yinkimbinkimbi yamaseli amagciwane wesilisa nowesifazane, kanye nemibungu ngenhloso yokuyigcina isikhathi esingenamkhawulo. I-sperm, ama-oocyte kanye nemibungu yinto enamandla yokushisa (kuze kube ku--196 degrees Celsius) ku-nitrogen egazini.
Ngaphambi kokushisa, wonke umswakama ususwe kumaseli, ngoba uyabulala ngenkathi efriziwe. Gcina impahla efriziwe emigodini ekhethekile - izitsha ze-Dewar. Zonke izingxenye zibhalwe, imibungu igcinwa ngo-1-2 ku-vitro.
Iyini i-cryopreservation ye-sperm nama-oocyte?
I-sperm ingafakwa iqhwa uma i-IVF ihlelwe, kodwa ngosuku lokuthunjwa kwalo muntu ngeke abe semtholampilo ngesinye isizathu. Isifo esithile esibi ngesinye isizathu sokusebenzisa ukuchotshozwa kwe-spermatozoa. Lokhu kwenzeke ezinyathelweni eziningana ukuqoqa inani elifanele lomdanso futhi kufeze ngempumelelo uhlelo lwe-in vitro fertilization.
Iqanda lingenziwa iqhwa kulabo abanesifo se-oncological. Ngaphambi kwemisebe ye-radiotherapy kanye ne-chemotherapy, okuvame ukuholela ekulahlekelweni kokuzala kwabesifazane , ungenza isitoreji samaqanda ukuze esikhathini esizayo abe nezingane.
Isinkwa esinamafutha
Iqoqwe ukuze iqothule isheke sowesilisa. Ihlushwa isilingo sokuqhathaniswa nokuvivinya. Uma izinkomba zilungile, futhi ukuhlolwa kokuthi kungenzeka yini ukufisa isidoda siphumelele, indaba eqoqwayo isanda futhi ikhulise ukuhlushwa, isetshenziswe kahle futhi ifakwe kwisitsha. Isiqukathi siyithambo elincane elincane leplastiki elincane. Le tube itholakala ukuze kungabikho iphutha uma kuhlanganiswa ne-semen.
I-Cryopreservation yama-oocyte
Ukuqoqwa kwamaqanda ngokuqandisa kuyinkqubo ende futhi eyinkimbinkimbi kunesidoda. Owesifazane unikezwa ukugqugquzelwa kwama-ovari ukuze ama-oocyte amaningana avuthiwe ngesikhathi esifanayo. Emva kwalokhu, kukhona ukukhishwa kwamaqanda, ukukhethwa kwezinto ezinamandla kakhulu. Zilashwa futhi uketshezi okwedlulele ususwe, bese ufakwa esitsheni esikhethekile futhi efriziwe nge-nitrogen egazini.
Cryopreservation yemibungu
Imibungu inqamule ngezinhloso eziningana. Okokuqala, ukuze, uma kwenzeka umzamo ongaphumelelanga, i-IVF ingabuye ihlolwe futhi ngaphandle kokuvuselela ama-ovari nokugubha ama-oocyte.
Ngaphezu kwalokho, ama-embrys awela ngaphansi kwe-cryopreservation ezimweni lapho owesifazane engabekezelelanga ukudluliselwa kwemibungu (i-ovarian hyperstimulation syndrome). Ukungaqhathaniswa kwe-endometrium kungenye isizathu semibungu yokuqandisa. Imibungu kulokhu ihlakulela izinsuku ezimbalwa, ngakho-ke ama-best of them akhethiwe futhi angenwa amandla okushisa. Lapho i-endometrium yowesifazane isilungele imibungu, ukudluliselwa kwemibungu eqhwayo kwenzeka.
Umbungu ogcweleyo ugcinwa uma nje ufuna. Yiqiniso, inqubo yokuqhwaza nokuqeda ukucindezeleka emibungu. Kodwa izindlela zanamuhla azivumeli nje ukugcina inani elikhulu lemibungu eqhwayo esimweni esisebenzayo, kodwa nokuqinisekisa ukuthi zivamile
Izimo ze-cryopreservation yemibungu
Okokuqala, imibungu engcono kuphela inika isithwathwa - enezinkomba eziphezulu kakhulu zekhwalithi. Okwesibili, kwenzeka ngesiteji esithile sokuthuthukiswa kwabo: ngezigaba 2, 4, 8 kanye nama-blastocysts.
Imibungu, enezinkomba zayo ezichazwa njengezimbi, ungaziboleki ekuqandeni, njengoba zinendawo yokuhlambalaza - ukwehla. Ngezinye izikhathi imibungu emihle nayo ibhujiswe - lena yindleko yokuqhaqhazela kwabo futhi okulandelayo. Kodwa kunama-embryos amaningana amaqanda, ngakho ezinye zazo zihlala zikwazi ukusebenza kahle ngemva kokufa.