Iyini i-cryoconservation of oocytes?
Le ndlela ibhekwa njengohlobo lobuchwepheshe bokuhlola. Into yukuthi ngokuvamile lapho iqhutshwa, izinga lokusinda kwama-oocyte ngemuva kokuqandisa liphansi kakhulu. Ngaphezu kwalokho, amangqamuzana ezocansi, emva kokuthungula nokubeka emithonjeni yezokudla, akakwazi ukukhulelwa njalo.
Ukuphumelela kwalendlela kungalungiswa kuphela uma owesifazane engenaye umlingani wesondo noma engakakulungeli ukuba umama. Ezimweni ezifanayo, lokhu mhlawumbe kuphela ithuba lokukhulelwa futhi ube nomntwana. Njengezithunywa ezisetshenziselwa ukukhipha ama-oocyte, ama-cryoprotectants afana ne-ethylene glycol ne-dimethylsulfoxide angenza. Ukwelashwa kwamaqanda nakho kungenziwa ngendlela efanayo . Kumele kuqashelwe ukuthi isikhathi sokugcina asithinti ukusinda nganoma iyiphi indlela.
Konke kuncike ekutheni okuthiwa yi-oocyte eyinkimbinkimbi yesakhiwo. Ngakho-ke, ngaphambi kokwenza inqubo enjalo, ukhetho olubalulekile lukhetha ngokucophelela, olwenziwe ngokuhlola ama-oocyte ku-microscope ekhethekile.
Ngokwezibalo zokubheka, izinga lokusinda kwama-oocyte afriziwe cishe cishe angu-68%, kanti imvamisa yokukhulelwa kwabo ingama-48%. Uma sikhuluma ngokuphindaphindiwe kokubona kokukhulelwa kwe-oocyte ngayinye efriziwe, lokhu kubonwe ngamacala angu-2%.
Iyini i-cryopreservation ye-embryo?
Lolu hlobo lokuqhwaza kwe-biomaterial ngenqubo ye-IVF elandelayo luyaqhubeka phambili. Into yokuthi imibungu ye-cryopreservation inikeza okungcono kakhulu.
Ukusetshenziswa kwale nqubo kuvumela inqubo yokufaka umanyolo in vitro kwenziwe ngomjikelezo owodwa. Ngakho-ke, uma kwenzeka ukuthi ngemva kokufakelwa kokukhulelwa komzimba okhulelwe, ungasebenzisa i-cryopreserved, futhi ungahlakuleli okusha kumaphakathi wezakhi.
- ukunciphisa isikhathi kanye nezindleko zempahla ye-IVF (izakhamzimba ezakhiwa izakhi ezibiza kakhulu);
- ukunciphisa isikhathi senkambiso;
- ithuba lokuphinda i-IVF ngemva kwesikhathi eside.
Izithiyo eziyinhloko zalolu daba zifakazela ukuthi amathuba okukhulelwa angama-60%, kanti izinga lokusinda lemibungu ngemuva kokukhishwa kwazo kunezinhlobo eziningi zokuhamba, kusukela ku-35 kuya ku-90%. Njengoba kunikezwe la maqiniso, kunzima ukubikezela ukuthi imibungu izobe isetshenziselwa kanjani emva kwe-cryopreservation.